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noggin atcc crl  (ATCC)


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    ATCC noggin atcc crl
    Noggin Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/noggin+atcc+crl/L-WRN/pm41722050-802-144-145
    Average 98 stars, based on 482 article reviews
    noggin atcc crl - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Control:

    Article Title: Cytosolic DNA composition is determined by genomic instability mechanism and regulates dendritic cell-mediated anti-tumor immunity.
    Article Snippet: In brief Mosley et al. find that strongly immunogenic cancers with MSI produce cytosolic DNA (cyDNA) more effective at promoting STING-mediated anti-tumor immune responses than those with CIN.. Highly stimulatory cyDNAs produced by MSI and DNA-damaging therapies contain specific features, including microsatellites, increased size, and mitochondrial cyDNA, that strongly activate STING and enhance anti-tumor immunity.

    Virus:

    Article Title: Cytosolic DNA composition is determined by genomic instability mechanism and regulates dendritic cell-mediated anti-tumor immunity.
    Article Snippet: In brief Mosley et al. find that strongly immunogenic cancers with MSI produce cytosolic DNA (cyDNA) more effective at promoting STING-mediated anti-tumor immune responses than those with CIN.. Highly stimulatory cyDNAs produced by MSI and DNA-damaging therapies contain specific features, including microsatellites, increased size, and mitochondrial cyDNA, that strongly activate STING and enhance anti-tumor immunity.

    Article Title: Cytosolic DNA structures produced by mismatch repair deficiency coordinate anti-tumor immunity in colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies G4 (BG4) Sigma Millipore Cat# MABE917; RRID:AB_2750936 Ki67 PECy5 eBioscience Cat# 15-5698-82; RRID:AB_2802209 CD8 FITC eBioscience Cat# 11-0081-82; RRID:AB_464915 CCR5 APC eBioscience Cat# 17-1951-82; RRID:AB_2802223 pTBK1 Cell Signaling Technology Cat# 5483S; RRID:AB_10693472 TBK1 Cell Signaling Technology Cat# 3504S; RRID:AB_2255663 pSTAT1 Thermo Fisher Cat# 333400; RRID:AB_2533113 STAT1 Cell Signaling Technology Cat# 9172S; RRID:AB_2198300 GAPDH Thermo Fisher Cat# PIMA515738; RRID:AB_10977387 cGAS Cell Signaling Technology Cat# 31659; RRID:AB_2799008 CD69 A700 eBioscience Cat# 56-0691-82; RRID:AB_2815240 PD1 SB600 eBioscience Cat# 63-9981-82; RRID:AB_2744860 IL10 PE eBioscience Cat# 12-7101-82; RRID:AB_466176 GITR PECy7 eBioscience Cat# 25-5874-82; RRID:AB_10548516 IFNγ PE eBioscience Cat# 12-7311-82; RRID:AB_466193 CD4 FITC eBioscience Cat# 11-0041-85; RRID:AB_464893 FOXP3 APC eBioscience Cat# 17-5773-82; RRID:AB_469457 Rat IgG Blocking Antibody BioXCell Cat# BE0089; RRID:AB_1107769 IL10 Blocking Antibody (JES5-2A5) BioXCell Cat# BE0049; RRID:AB_1107696 Bacterial and virus strains pLKO.1 Addgene 24150; RRID:Addgene_24150 Biological samples L-cells expressing Wnt3a, R-spondin and Noggin ATCC CRL-3276; RRID:CVCL_DA06 Chemicals, peptides, and recombinant proteins Ammonium chloride Fisher Scientific A661-500 Potassium bicarbonate Fisher Scientific P184-500 EDTA Fisher Scientific BP2482-1 MCSF StemCell 78057 Trypsin Gibco 25200–072 Digitonin Millipore Sigma 11024-24-1 Hexylene Glycol Fisher Scientific AAL03187AP Proteinase K Fisher Scientific BP1700-500 RNase A Thermo Fisher Scientific EN0531 Phenol/Chloroform/Isoamyl Alcohol Fisher Scientific BP1752I-400 P1 Nuclease NEB M0660S NEB Buffer 1.1 NEB B7201S Lithium chloride Fisher Scientific L121-100 Lipofectamine 2000 Thermo Fisher Scientific 11668019 Herring Sperm DNA (HT-DNA) Thermo Fisher Scientific 15634–017 PicoGreen Thermo Fisher Scientific P7581 DAPI Thermo Fisher Scientific D1306 Ovalbumin Protein Millipore Sigma A5378 PMA Millipore Sigma P1585 (Continued on next page) Cell Reports 45, 117012, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Thermo Fisher J60628 Monensin Thermo Fisher 00-4505-51 Bovine Serum Albumin StemCell 100–0175 Na-deoxycholate Fisher Scientific BP349-100 Calcium chloride Fisher Scientific BP510-500 cGAS Protein Cayman Chemicals 22810 SYBR Gold Thermo Fisher Scientific S11494 Rat Serum StemCell 13551 Triton X-100 Fisher Scientific BP151-500 Cytochalasin D StemCell 100–0556 Nuclei Extraction Buffer Miltenyi Biotec 130-128-024 Sucrose Fisher Scientific BP220-1 Magnesium acetate Alfa Aesar 36358 DTT Fisher Scientific BP172-25 Spermine Alfa Aesar L19562 Spermidine Alfa Aesar A19096 Dextran Sulfate Sodium (DSS) Sigma-Aldrich J63606 DNase I Thermo Fisher Scientific D2821 Collagenase IV Sigma Aldrich C5138-5G TRIzol Thermo Fisher Scientific 15596026 PMA Millipore Sigma P1585 Ionomycin Thermo Fisher Scientific J60628 Monensin Thermo Fisher Scientific 00-4505-51 Collagenase XI Millipore Sigma C5138 Dispase II Millipore Sigma D4693 Matrigel Growth Factor Reduced (GFR) Corning 354230 N-Acetylcystein Thermo Fisher Scientific 160280250 N2 Supplement Thermo Fisher Scientific 17502048 B27 Supplement Thermo Fisher Scientific 17504044 Nicotinamide Millipore Sigma N0636 A83-01 Millipore Sigma SML0788 SB202190 Millipore Sigma S7067 EGF Thermo Fisher Scientific PMG8043 TrypLE Express Thermo Fisher Scientific 12605028 Polybrene Sigma-Adrich H9268 Y27632 Sigma-Aldrich Y0503 Hygromycin Thermo Fisher Scientific 10687010 Cell Recovery Solution Corning 354253 NEB Buffer 2 NEB B7002S Critical commercial assays EasySep Mouse CD8 + T cell Isolation Kit StemCell 19853A Zombie Aqua Fixable Viability Kit BioLegend 423102 FOXP3 Transcription Factor Staining Kit eBioscience 00-5523-00 Qubit RNA BR Kit Thermo Fisher Scientific Q10210 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368814 PowerUp SYBR Green Master Mix Applied Biosystems A25742 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 (Continued on next page) 18 Cell Reports 45, 117012, March 24, 2026

    Expressing:

    Article Title: Cytosolic DNA composition is determined by genomic instability mechanism and regulates dendritic cell-mediated anti-tumor immunity.
    Article Snippet: In brief Mosley et al. find that strongly immunogenic cancers with MSI produce cytosolic DNA (cyDNA) more effective at promoting STING-mediated anti-tumor immune responses than those with CIN.. Highly stimulatory cyDNAs produced by MSI and DNA-damaging therapies contain specific features, including microsatellites, increased size, and mitochondrial cyDNA, that strongly activate STING and enhance anti-tumor immunity.

    Article Title: Cytosolic DNA structures produced by mismatch repair deficiency coordinate anti-tumor immunity in colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies G4 (BG4) Sigma Millipore Cat# MABE917; RRID:AB_2750936 Ki67 PECy5 eBioscience Cat# 15-5698-82; RRID:AB_2802209 CD8 FITC eBioscience Cat# 11-0081-82; RRID:AB_464915 CCR5 APC eBioscience Cat# 17-1951-82; RRID:AB_2802223 pTBK1 Cell Signaling Technology Cat# 5483S; RRID:AB_10693472 TBK1 Cell Signaling Technology Cat# 3504S; RRID:AB_2255663 pSTAT1 Thermo Fisher Cat# 333400; RRID:AB_2533113 STAT1 Cell Signaling Technology Cat# 9172S; RRID:AB_2198300 GAPDH Thermo Fisher Cat# PIMA515738; RRID:AB_10977387 cGAS Cell Signaling Technology Cat# 31659; RRID:AB_2799008 CD69 A700 eBioscience Cat# 56-0691-82; RRID:AB_2815240 PD1 SB600 eBioscience Cat# 63-9981-82; RRID:AB_2744860 IL10 PE eBioscience Cat# 12-7101-82; RRID:AB_466176 GITR PECy7 eBioscience Cat# 25-5874-82; RRID:AB_10548516 IFNγ PE eBioscience Cat# 12-7311-82; RRID:AB_466193 CD4 FITC eBioscience Cat# 11-0041-85; RRID:AB_464893 FOXP3 APC eBioscience Cat# 17-5773-82; RRID:AB_469457 Rat IgG Blocking Antibody BioXCell Cat# BE0089; RRID:AB_1107769 IL10 Blocking Antibody (JES5-2A5) BioXCell Cat# BE0049; RRID:AB_1107696 Bacterial and virus strains pLKO.1 Addgene 24150; RRID:Addgene_24150 Biological samples L-cells expressing Wnt3a, R-spondin and Noggin ATCC CRL-3276; RRID:CVCL_DA06 Chemicals, peptides, and recombinant proteins Ammonium chloride Fisher Scientific A661-500 Potassium bicarbonate Fisher Scientific P184-500 EDTA Fisher Scientific BP2482-1 MCSF StemCell 78057 Trypsin Gibco 25200–072 Digitonin Millipore Sigma 11024-24-1 Hexylene Glycol Fisher Scientific AAL03187AP Proteinase K Fisher Scientific BP1700-500 RNase A Thermo Fisher Scientific EN0531 Phenol/Chloroform/Isoamyl Alcohol Fisher Scientific BP1752I-400 P1 Nuclease NEB M0660S NEB Buffer 1.1 NEB B7201S Lithium chloride Fisher Scientific L121-100 Lipofectamine 2000 Thermo Fisher Scientific 11668019 Herring Sperm DNA (HT-DNA) Thermo Fisher Scientific 15634–017 PicoGreen Thermo Fisher Scientific P7581 DAPI Thermo Fisher Scientific D1306 Ovalbumin Protein Millipore Sigma A5378 PMA Millipore Sigma P1585 (Continued on next page) Cell Reports 45, 117012, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Thermo Fisher J60628 Monensin Thermo Fisher 00-4505-51 Bovine Serum Albumin StemCell 100–0175 Na-deoxycholate Fisher Scientific BP349-100 Calcium chloride Fisher Scientific BP510-500 cGAS Protein Cayman Chemicals 22810 SYBR Gold Thermo Fisher Scientific S11494 Rat Serum StemCell 13551 Triton X-100 Fisher Scientific BP151-500 Cytochalasin D StemCell 100–0556 Nuclei Extraction Buffer Miltenyi Biotec 130-128-024 Sucrose Fisher Scientific BP220-1 Magnesium acetate Alfa Aesar 36358 DTT Fisher Scientific BP172-25 Spermine Alfa Aesar L19562 Spermidine Alfa Aesar A19096 Dextran Sulfate Sodium (DSS) Sigma-Aldrich J63606 DNase I Thermo Fisher Scientific D2821 Collagenase IV Sigma Aldrich C5138-5G TRIzol Thermo Fisher Scientific 15596026 PMA Millipore Sigma P1585 Ionomycin Thermo Fisher Scientific J60628 Monensin Thermo Fisher Scientific 00-4505-51 Collagenase XI Millipore Sigma C5138 Dispase II Millipore Sigma D4693 Matrigel Growth Factor Reduced (GFR) Corning 354230 N-Acetylcystein Thermo Fisher Scientific 160280250 N2 Supplement Thermo Fisher Scientific 17502048 B27 Supplement Thermo Fisher Scientific 17504044 Nicotinamide Millipore Sigma N0636 A83-01 Millipore Sigma SML0788 SB202190 Millipore Sigma S7067 EGF Thermo Fisher Scientific PMG8043 TrypLE Express Thermo Fisher Scientific 12605028 Polybrene Sigma-Adrich H9268 Y27632 Sigma-Aldrich Y0503 Hygromycin Thermo Fisher Scientific 10687010 Cell Recovery Solution Corning 354253 NEB Buffer 2 NEB B7002S Critical commercial assays EasySep Mouse CD8 + T cell Isolation Kit StemCell 19853A Zombie Aqua Fixable Viability Kit BioLegend 423102 FOXP3 Transcription Factor Staining Kit eBioscience 00-5523-00 Qubit RNA BR Kit Thermo Fisher Scientific Q10210 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368814 PowerUp SYBR Green Master Mix Applied Biosystems A25742 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 (Continued on next page) 18 Cell Reports 45, 117012, March 24, 2026

    Recombinant:

    Article Title: Cytosolic DNA composition is determined by genomic instability mechanism and regulates dendritic cell-mediated anti-tumor immunity.
    Article Snippet: In brief Mosley et al. find that strongly immunogenic cancers with MSI produce cytosolic DNA (cyDNA) more effective at promoting STING-mediated anti-tumor immune responses than those with CIN.. Highly stimulatory cyDNAs produced by MSI and DNA-damaging therapies contain specific features, including microsatellites, increased size, and mitochondrial cyDNA, that strongly activate STING and enhance anti-tumor immunity.

    Article Title: Cytosolic DNA structures produced by mismatch repair deficiency coordinate anti-tumor immunity in colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies G4 (BG4) Sigma Millipore Cat# MABE917; RRID:AB_2750936 Ki67 PECy5 eBioscience Cat# 15-5698-82; RRID:AB_2802209 CD8 FITC eBioscience Cat# 11-0081-82; RRID:AB_464915 CCR5 APC eBioscience Cat# 17-1951-82; RRID:AB_2802223 pTBK1 Cell Signaling Technology Cat# 5483S; RRID:AB_10693472 TBK1 Cell Signaling Technology Cat# 3504S; RRID:AB_2255663 pSTAT1 Thermo Fisher Cat# 333400; RRID:AB_2533113 STAT1 Cell Signaling Technology Cat# 9172S; RRID:AB_2198300 GAPDH Thermo Fisher Cat# PIMA515738; RRID:AB_10977387 cGAS Cell Signaling Technology Cat# 31659; RRID:AB_2799008 CD69 A700 eBioscience Cat# 56-0691-82; RRID:AB_2815240 PD1 SB600 eBioscience Cat# 63-9981-82; RRID:AB_2744860 IL10 PE eBioscience Cat# 12-7101-82; RRID:AB_466176 GITR PECy7 eBioscience Cat# 25-5874-82; RRID:AB_10548516 IFNγ PE eBioscience Cat# 12-7311-82; RRID:AB_466193 CD4 FITC eBioscience Cat# 11-0041-85; RRID:AB_464893 FOXP3 APC eBioscience Cat# 17-5773-82; RRID:AB_469457 Rat IgG Blocking Antibody BioXCell Cat# BE0089; RRID:AB_1107769 IL10 Blocking Antibody (JES5-2A5) BioXCell Cat# BE0049; RRID:AB_1107696 Bacterial and virus strains pLKO.1 Addgene 24150; RRID:Addgene_24150 Biological samples L-cells expressing Wnt3a, R-spondin and Noggin ATCC CRL-3276; RRID:CVCL_DA06 Chemicals, peptides, and recombinant proteins Ammonium chloride Fisher Scientific A661-500 Potassium bicarbonate Fisher Scientific P184-500 EDTA Fisher Scientific BP2482-1 MCSF StemCell 78057 Trypsin Gibco 25200–072 Digitonin Millipore Sigma 11024-24-1 Hexylene Glycol Fisher Scientific AAL03187AP Proteinase K Fisher Scientific BP1700-500 RNase A Thermo Fisher Scientific EN0531 Phenol/Chloroform/Isoamyl Alcohol Fisher Scientific BP1752I-400 P1 Nuclease NEB M0660S NEB Buffer 1.1 NEB B7201S Lithium chloride Fisher Scientific L121-100 Lipofectamine 2000 Thermo Fisher Scientific 11668019 Herring Sperm DNA (HT-DNA) Thermo Fisher Scientific 15634–017 PicoGreen Thermo Fisher Scientific P7581 DAPI Thermo Fisher Scientific D1306 Ovalbumin Protein Millipore Sigma A5378 PMA Millipore Sigma P1585 (Continued on next page) Cell Reports 45, 117012, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Thermo Fisher J60628 Monensin Thermo Fisher 00-4505-51 Bovine Serum Albumin StemCell 100–0175 Na-deoxycholate Fisher Scientific BP349-100 Calcium chloride Fisher Scientific BP510-500 cGAS Protein Cayman Chemicals 22810 SYBR Gold Thermo Fisher Scientific S11494 Rat Serum StemCell 13551 Triton X-100 Fisher Scientific BP151-500 Cytochalasin D StemCell 100–0556 Nuclei Extraction Buffer Miltenyi Biotec 130-128-024 Sucrose Fisher Scientific BP220-1 Magnesium acetate Alfa Aesar 36358 DTT Fisher Scientific BP172-25 Spermine Alfa Aesar L19562 Spermidine Alfa Aesar A19096 Dextran Sulfate Sodium (DSS) Sigma-Aldrich J63606 DNase I Thermo Fisher Scientific D2821 Collagenase IV Sigma Aldrich C5138-5G TRIzol Thermo Fisher Scientific 15596026 PMA Millipore Sigma P1585 Ionomycin Thermo Fisher Scientific J60628 Monensin Thermo Fisher Scientific 00-4505-51 Collagenase XI Millipore Sigma C5138 Dispase II Millipore Sigma D4693 Matrigel Growth Factor Reduced (GFR) Corning 354230 N-Acetylcystein Thermo Fisher Scientific 160280250 N2 Supplement Thermo Fisher Scientific 17502048 B27 Supplement Thermo Fisher Scientific 17504044 Nicotinamide Millipore Sigma N0636 A83-01 Millipore Sigma SML0788 SB202190 Millipore Sigma S7067 EGF Thermo Fisher Scientific PMG8043 TrypLE Express Thermo Fisher Scientific 12605028 Polybrene Sigma-Adrich H9268 Y27632 Sigma-Aldrich Y0503 Hygromycin Thermo Fisher Scientific 10687010 Cell Recovery Solution Corning 354253 NEB Buffer 2 NEB B7002S Critical commercial assays EasySep Mouse CD8 + T cell Isolation Kit StemCell 19853A Zombie Aqua Fixable Viability Kit BioLegend 423102 FOXP3 Transcription Factor Staining Kit eBioscience 00-5523-00 Qubit RNA BR Kit Thermo Fisher Scientific Q10210 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368814 PowerUp SYBR Green Master Mix Applied Biosystems A25742 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 (Continued on next page) 18 Cell Reports 45, 117012, March 24, 2026

    Blocking Assay:

    Article Title: Cytosolic DNA structures produced by mismatch repair deficiency coordinate anti-tumor immunity in colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies G4 (BG4) Sigma Millipore Cat# MABE917; RRID:AB_2750936 Ki67 PECy5 eBioscience Cat# 15-5698-82; RRID:AB_2802209 CD8 FITC eBioscience Cat# 11-0081-82; RRID:AB_464915 CCR5 APC eBioscience Cat# 17-1951-82; RRID:AB_2802223 pTBK1 Cell Signaling Technology Cat# 5483S; RRID:AB_10693472 TBK1 Cell Signaling Technology Cat# 3504S; RRID:AB_2255663 pSTAT1 Thermo Fisher Cat# 333400; RRID:AB_2533113 STAT1 Cell Signaling Technology Cat# 9172S; RRID:AB_2198300 GAPDH Thermo Fisher Cat# PIMA515738; RRID:AB_10977387 cGAS Cell Signaling Technology Cat# 31659; RRID:AB_2799008 CD69 A700 eBioscience Cat# 56-0691-82; RRID:AB_2815240 PD1 SB600 eBioscience Cat# 63-9981-82; RRID:AB_2744860 IL10 PE eBioscience Cat# 12-7101-82; RRID:AB_466176 GITR PECy7 eBioscience Cat# 25-5874-82; RRID:AB_10548516 IFNγ PE eBioscience Cat# 12-7311-82; RRID:AB_466193 CD4 FITC eBioscience Cat# 11-0041-85; RRID:AB_464893 FOXP3 APC eBioscience Cat# 17-5773-82; RRID:AB_469457 Rat IgG Blocking Antibody BioXCell Cat# BE0089; RRID:AB_1107769 IL10 Blocking Antibody (JES5-2A5) BioXCell Cat# BE0049; RRID:AB_1107696 Bacterial and virus strains pLKO.1 Addgene 24150; RRID:Addgene_24150 Biological samples L-cells expressing Wnt3a, R-spondin and Noggin ATCC CRL-3276; RRID:CVCL_DA06 Chemicals, peptides, and recombinant proteins Ammonium chloride Fisher Scientific A661-500 Potassium bicarbonate Fisher Scientific P184-500 EDTA Fisher Scientific BP2482-1 MCSF StemCell 78057 Trypsin Gibco 25200–072 Digitonin Millipore Sigma 11024-24-1 Hexylene Glycol Fisher Scientific AAL03187AP Proteinase K Fisher Scientific BP1700-500 RNase A Thermo Fisher Scientific EN0531 Phenol/Chloroform/Isoamyl Alcohol Fisher Scientific BP1752I-400 P1 Nuclease NEB M0660S NEB Buffer 1.1 NEB B7201S Lithium chloride Fisher Scientific L121-100 Lipofectamine 2000 Thermo Fisher Scientific 11668019 Herring Sperm DNA (HT-DNA) Thermo Fisher Scientific 15634–017 PicoGreen Thermo Fisher Scientific P7581 DAPI Thermo Fisher Scientific D1306 Ovalbumin Protein Millipore Sigma A5378 PMA Millipore Sigma P1585 (Continued on next page) Cell Reports 45, 117012, March 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Thermo Fisher J60628 Monensin Thermo Fisher 00-4505-51 Bovine Serum Albumin StemCell 100–0175 Na-deoxycholate Fisher Scientific BP349-100 Calcium chloride Fisher Scientific BP510-500 cGAS Protein Cayman Chemicals 22810 SYBR Gold Thermo Fisher Scientific S11494 Rat Serum StemCell 13551 Triton X-100 Fisher Scientific BP151-500 Cytochalasin D StemCell 100–0556 Nuclei Extraction Buffer Miltenyi Biotec 130-128-024 Sucrose Fisher Scientific BP220-1 Magnesium acetate Alfa Aesar 36358 DTT Fisher Scientific BP172-25 Spermine Alfa Aesar L19562 Spermidine Alfa Aesar A19096 Dextran Sulfate Sodium (DSS) Sigma-Aldrich J63606 DNase I Thermo Fisher Scientific D2821 Collagenase IV Sigma Aldrich C5138-5G TRIzol Thermo Fisher Scientific 15596026 PMA Millipore Sigma P1585 Ionomycin Thermo Fisher Scientific J60628 Monensin Thermo Fisher Scientific 00-4505-51 Collagenase XI Millipore Sigma C5138 Dispase II Millipore Sigma D4693 Matrigel Growth Factor Reduced (GFR) Corning 354230 N-Acetylcystein Thermo Fisher Scientific 160280250 N2 Supplement Thermo Fisher Scientific 17502048 B27 Supplement Thermo Fisher Scientific 17504044 Nicotinamide Millipore Sigma N0636 A83-01 Millipore Sigma SML0788 SB202190 Millipore Sigma S7067 EGF Thermo Fisher Scientific PMG8043 TrypLE Express Thermo Fisher Scientific 12605028 Polybrene Sigma-Adrich H9268 Y27632 Sigma-Aldrich Y0503 Hygromycin Thermo Fisher Scientific 10687010 Cell Recovery Solution Corning 354253 NEB Buffer 2 NEB B7002S Critical commercial assays EasySep Mouse CD8 + T cell Isolation Kit StemCell 19853A Zombie Aqua Fixable Viability Kit BioLegend 423102 FOXP3 Transcription Factor Staining Kit eBioscience 00-5523-00 Qubit RNA BR Kit Thermo Fisher Scientific Q10210 High-Capacity cDNA Reverse Transcription Kit Applied Biosystems 4368814 PowerUp SYBR Green Master Mix Applied Biosystems A25742 Pierce BCA Protein Assay Kit Thermo Fisher Scientific 23225 (Continued on next page) 18 Cell Reports 45, 117012, March 24, 2026



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